Determination of di-and polyamines by high-performance liquid chromatographic separation of their 5-dimethylaminonaphthalene-1-sulfonyl derivatives

Abstract

Using a Lichrosorb RP-8 reversed-phase column and a methanol--water gradient elution program, it is possible to separate within 40 min and to determine routinely in picomole quantities the natural di- and polyamines. The precision of the method is comparable to the thin-layer chromatographic procedures, the separations are most efficient, and the method can be fully automated. A modified gradient enables the repeated assay of spermidine and spermine within 20 min. The method is suited for polyamine analyses in tissues and body fluids.