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. 1982 Aug 1;205(2):285-93.
doi: 10.1042/bj2050285.

Purification and structural studies on the complement-system control protein beta 1H (Factor H)

R B Sim, R G DiScipio

Purification and structural studies on the complement-system control protein beta 1H (Factor H)

R B Sim et al. Biochem J. .

Abstract

An efficient procedure for the isolation of the complement-system control protein beta 1H (Factor H) from human plasma was developed. The chemical composition and physical characteristics of the protein were studied, and a sequence of 17 amino acid residues at the N-terminus was determined. Factor H is a single-polypeptide-chain glycoprotein of mol.wt. 155 000 containing 9.3% carbohydrate. Factor H is cleaved by plasma proteinases to a two-chain form. This cleavage can be mimicked by trypsin, and the two-chain form retains fully the C3b-inactivator cofactor activity of Factor H. The proteolytic fragments of Factor H are compared with those of other proteins (C4b-binding protein and erythrocyte C3b-receptor) that act as cofactors for C3b-inactivator.

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References

    1. J Exp Med. 1978 Jun 1;147(6):1792-1805 - PubMed
    1. J Exp Med. 1979 Aug 1;150(2):267-76 - PubMed
    1. Mol Immunol. 1979 Oct;16(10):767-76 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5867-71 - PubMed
    1. Clin Exp Immunol. 1979 Dec;38(3):397-404 - PubMed