Excision of apurinic sites from DNA with enzymes isolated from rat-liver chromatin
- PMID: 6217070
- DOI: 10.1111/j.1432-1033.1982.tb06917.x
Excision of apurinic sites from DNA with enzymes isolated from rat-liver chromatin
Abstract
Apurinic sites were excised from phi X174 RF DNA with two enzymes isolated from rat liver chromatin: an apurinic/apyrimidinic endodeoxyribonuclease and a 5'-3'-exonuclease; the resulting gap was filled with DNA polymerase beta also prepared from rat liver chromatin and the repair was fully terminated with T4 ligase.
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