Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Aug;19(2):1213-7.
doi: 10.1016/0022-4731(83)90419-3.

Active site-directed irreversible inhibition of 3 beta-hydroxysteroid dehydrogenase from human placenta

Y Chikhaoui, A M Boussioux, J C Nicolas, B Descomps, A Crastes de Paulet

Active site-directed irreversible inhibition of 3 beta-hydroxysteroid dehydrogenase from human placenta

Y Chikhaoui et al. J Steroid Biochem. 1983 Aug.

Abstract

To obtain a placental microsome preparation able to convert androstenedione and testosterone specifically to estrogens, 3 beta-hydroxysteroid dehydrogenase must be eliminated. After solubilisation by Triton X-100, the remaining 3 beta-hydroxysteroid dehydrogenase activity is inhibited by 11 alpha-bromoacetoxyprogesterone, an alkylating analogue of progesterone, which behaves as an irreversible active site-directed inhibitor. The enzyme is protected against inactivation and alkylation by steroid and coenzyme substrates. The inhibition is specific to 3 beta-hydroxysteroid dehydrogenase and the estrogen synthetase (aromatase) activity contained in the preparation is not affected by this inhibitor.

PubMed Disclaimer