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Comparative Study
. 1983;192(1-2):10-4.
doi: 10.1007/BF00327640.

Genetic characterization of a gene for prolipoprotein signal peptidase in Escherichia coli

H Yamagata, N Taguchi, K Daishima, S Mizushima
Comparative Study

Genetic characterization of a gene for prolipoprotein signal peptidase in Escherichia coli

H Yamagata et al. Mol Gen Genet. 1983.

Abstract

A mutation (lspA, prolipoprotein signal peptidase) rendering the prolipoprotein signal peptidase temperature-sensitive in Escherichia coli has been analyzed. The mutation was mapped in the dnaJ-rpsT-ileS-dapB region by interrupted mating with various Hfr strains and P1 phage transduction. lambda transducing phage lambda ddapB2 that carries the rpsT-ileS-dapB region was shown to complement the lspA mutation. Plasmid pLC3-13 which had been isolated from Clarke and Carbon's collection as a plasmid carrying the lspA locus was shown to carry the dnaJ and rpsT loci. Complementation analysis with plasmids carrying various DNA fragments derived from pLC3-13 showed that the lspA locus is between the rpsT and ileS loci. The wildtype allele was dominant over the lspA allele.

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References

    1. J Bacteriol. 1980 Aug;143(2):661-7 - PubMed
    1. J Biol Chem. 1980 Aug 25;255(16):7973-7 - PubMed
    1. Biomembranes. 1979;10:141-208 - PubMed
    1. Mol Gen Genet. 1978 Aug 4;164(1):9-14 - PubMed
    1. Microbiol Rev. 1980 Mar;44(1):1-56 - PubMed

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