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Comparative Study
. 1983 Dec 10;11(23):8495-508.
doi: 10.1093/nar/11.23.8495.

A chicken repetitive DNA sequence that is highly sensitive to single-strand specific endonucleases

K Dybvig, C D Clark, G Aliperti, M J Schlesinger
Free PMC article
Comparative Study

A chicken repetitive DNA sequence that is highly sensitive to single-strand specific endonucleases

K Dybvig et al. Nucleic Acids Res. .
Free PMC article

Abstract

A DNA sequence consisting of the 5-mer AGAGG repeated tandemly 32 times has been detected in a chicken genomic clone and found to be present in about 2000 copies per chicken genome. This sequence was highly susceptible to single-strand specific endonucleases isolated from Aspergillus oryzae (S1) and mung bean, but cleavage by a single-strand specific endonuclease isolated from Neurospora crassa occurred only at a pH below 5.5. Endonucleolytic cutting of the AGAGG sequence by the single-strand specific enzymes required a supercoiled substrate and was independent of ionic strength.

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