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. 1984 Apr;3(4):847-53.
doi: 10.1002/j.1460-2075.1984.tb01895.x.

Differential binding of a S. cerevisiae RNA polymerase III transcription factor to two promoter segments of a tRNA gene

D J Stillman, E P Geiduschek

Differential binding of a S. cerevisiae RNA polymerase III transcription factor to two promoter segments of a tRNA gene

D J Stillman et al. EMBO J. 1984 Apr.

Abstract

A Saccharomyces cerevisiae protein fraction which binds specifically to the internal promoter regions of genes that are transcribed by RNA polymerase III is shown to function as a transcription factor. We postulate that the stable DNA binding of the factor confers stability on polymerase III transcription complexes. Analysis of the binding by DNase 'foot-printing' distinguishes three segments of the S. cerevisiae tRNALeu3 gene: a region surrounding the so-called A block of the internal promoter, a region surrounding the B block and an intermediate segment. Binding to the A and B block regions is connected, but the B block region exerts a dominant effect.

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References

    1. Cell. 1983 Oct;34(3):881-90 - PubMed
    1. J Biol Chem. 1983 Feb 25;258(4):2440-6 - PubMed
    1. Curr Top Dev Biol. 1983;18:59-88 - PubMed
    1. Cell. 1982 Dec;31(2 Pt 1):319-25 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6191-5 - PubMed

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