1H-NMR study of the lambda operator site OL1: assignment of the imino and adenine H2 resonances
- PMID: 6233536
- PMCID: PMC318809
- DOI: 10.1093/nar/12.9.4035
1H-NMR study of the lambda operator site OL1: assignment of the imino and adenine H2 resonances
Abstract
One- and two-dimensional proton NMR methods are being used to study the synthetic lambda operator site O-L1, a 17 base-pair DNA duplex recognized by lambda repressor and Cro protein. The complete assignment of the 17 imino protons, which participate in Watson-Crick hydrogen bonding, and of the eight adenine H2 protons, which lie in the minor groove of the double helix, is presented.
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