doi: 10.1073/pnas.73.10.3463.
Human somatotropin: biological characterization of the recombinant molecule
- PMID: 62361
- PMCID: PMC431135
- DOI: 10.1073/pnas.73.10.3463
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Human somatotropin: biological characterization of the recombinant molecule
Proc Natl Acad Sci U S A.
1976 Oct.
Abstract
The recombinant hormone obtained by non-covalent interaction of the NH2-terminal 134 amino acid fragment with the COOH-terminal 51 amino acid fragment of the reduced-carbamidomethylated human somatotropin molecule is found to exhibit nearly full biological activity of the native hormone, as evidenced by the stimulation of hepatic ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) in vivo and protein synthesis in mouse mammary gland in vitro. Radioimmunoassay data indicate that the recombinant behaves immunochemically in a manner almost identical to that of the native hormone.
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