An efficient exchange method for alkali light chain in myosin subfragment

Abstract

The alkali light chain, A2, in subfragment-1 (S-1) was exchanged with A1 added externally in NH4 + -NH3 buffer (pH 9.9). The exchange yield was higher than 80% using only 2-fold molar excess of A1 over S-1 containing A2. The ATPase activities of the exchanged S-1 (A1) were the same as those of untreated S-1 (A1). The method was also applicable to exchanging the alkali light chains in myosin.