[Total activity and distribution of multiple forms of lysosomal glycosidases in subfractions of human leukocytes]

Abstract

Activities of N-acetyl-beta-D-hexosaminidase, beta-D-galactosidase, alpha-D-mannosidase and alpha-L-fucosidase were studied in granulocytes and agranulocytes of human blood. Hexosaminidase exhibited the highest activity in the both leukocyte fractions. At the same time, activities of hexosaminidase and galactosidase were higher in a granulocytes. Activity of mannosidase was higher in granulocytes. Activity of fucosidase was similar in the both subfractions. The following results were obtained after isoelectric focusing of hexosaminidase and fucosidase isoenzymes in polyacrylamide gel. Only one form, hexosaminidase A, was found in granulocytes, whereas two enzyme forms--hexosaminidase A (main component) and hexosaminidase B (minor fraction) were noted in agranulocytes. From 4 to 5 isoenzymes of fucosidase, detected in various human and animal tissues, were observed in the both leukocyte subfractions. Specific properties of the lysosomal hydrolases activity and of their isozyme spectra in the leukocyte subfractions are discussed.