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. 1984;14(5):485-97.
doi: 10.1080/00327488408061782.

Isolation of in vitro synthesized covalently closed circular double-stranded DNA by selective denaturation and filtration

S Shenoy, M Ehrlich

Isolation of in vitro synthesized covalently closed circular double-stranded DNA by selective denaturation and filtration

S Shenoy et al. Prep Biochem. 1984.

Abstract

Based upon their resistance to irreversible denaturation, covalently closed circular (CCC) DNA (non-nicked, double-stranded circular molecules) can be purified by alkaline denaturation, neutralization, and filtration through a nitrocellulose membrane. This procedure offers a simple means of isolating in vitro synthesized CCC DNA molecules. The preparations of molecules obtained by this method consisted of 91-97% CCC DNA and contained no detectable inhibitors of biological activity or enzymatic digestion.

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