Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980;25(1):40-9.
doi: 10.1007/BF02876396.

Streptococcal extracellular NAD+ nucleosidase. Characterization of changes occurring during purification

F J Zahradník

Streptococcal extracellular NAD+ nucleosidase. Characterization of changes occurring during purification

F J Zahradník. Folia Microbiol (Praha). 1980.

Abstract

Purification of streptococcal extracellular NAD+ nucleosidase is associated with changes of kinetic properties. A high-molecular weight component is required for a full activity of the enzyme. The component is not produced by bacteria and is present in the Todd-Hewitt cultivation medium, the beef-heart extract serving primarily as its source. In its effect on the enzyme this component can be replaced with bovine albumin. It follows from the time relationship of the NAD decomposition that the mechanism of the studied enzymic reaction is not hydrolytic. In addition to nicotinamide, a product of polymeric character is formed.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. J Exp Med. 1956 Oct 1;104(4):577-87 - PubMed
    1. J Biol Chem. 1967 Jul 10;242(13):3164-71 - PubMed
    1. J Hyg Epidemiol Microbiol Immunol. 1970;14(1):123-4 - PubMed
    1. J Biol Chem. 1969 Apr 10;244(7):1838-45 - PubMed
    1. J Biol Chem. 1970 Apr 10;245(7):1626-31 - PubMed

LinkOut - more resources