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. 1980 Feb 28;101(2-3):287-91.
doi: 10.1016/0009-8981(80)90255-7.

A direct, rapid, radioassay for adenosine diphosphatase

G Smith, G D Smith, T J Peters

A direct, rapid, radioassay for adenosine diphosphatase

G Smith et al. Clin Chim Acta. .

Abstract

Radiolabelled adenosine diphosphate [beta 32Pi]ADP was synthesized from radioactive orthophosphate and adenosine-5'-monophosphate with dicyclohexylcarbodiimide. The purified radioactive adenosine diphosphate was used as the substrate in a rapid, direct, one-step assay for adenosine diphosphatase in human neutrophilic leukocyte homogenates. Certain kinetic properties of the enzyme activity have been determined.

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