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. 1980 Jan;77(1):196-200.
doi: 10.1073/pnas.77.1.196.

A two-subunit cytochrome c oxidase (cytochrome aa3) from Paracoccus dentrificans

B Ludwig, G Schatz

A two-subunit cytochrome c oxidase (cytochrome aa3) from Paracoccus dentrificans

B Ludwig et al. Proc Natl Acad Sci U S A. 1980 Jan.

Abstract

Cytochrome c oxidase (ferrocytochrome c: oxygen oxidoreductase, EC 1.9.3.1) was purified from the cytoplasmic membrane of the bacterium Paracoccus denitrificans. The enzyme contains two heme groups (a and a3) and two copper atoms per minimal unit, oxidizes mammalian cytochrome c at a high rate, and, when incorporated into liposomes, generates an electrochemical proton gradient during cytochrome c oxidation. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis reveals only two subunits of apparent molecular weights 45,000 and 28,000; they appear to correspond to the two largest mitochondrially made subunits of the seven-subunit cytochrome c oxidase isolated from yeast mitochondria. Because of its structural simplicity. Paracoccus cytochrome c oxidase offers new possibilities for exploring the mechanism of cytochrome c oxidase function.

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References

    1. J Biol Chem. 1973 Mar 25;248(6):2049-55 - PubMed
    1. J Biol Chem. 1973 Feb 25;248(4):1346-54 - PubMed
    1. J Biol Chem. 1974 Dec 10;249(23):7390-401 - PubMed
    1. J Biol Chem. 1975 Jan 25;250(2):752-61 - PubMed
    1. Anal Biochem. 1976 Jan;70(1):263-7 - PubMed

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