doi: 10.1073/pnas.77.1.262.
Isolation of a replication origin complex from Escherichia coli
- PMID: 6244549
- PMCID: PMC348249
- DOI: 10.1073/pnas.77.1.262
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Isolation of a replication origin complex from Escherichia coli
Proc Natl Acad Sci U S A.
1980 Jan.
Abstract
A complex consisting of replicative origin DNA and several proteins was isolated from Escherichia coli. Cells of temperature-sensitive mutants were labeled at the origin and fractionated by sucrose gradient centrifugation. A complex highly purified in origin DNA sedimented as a unique band. This complex dissociated at high concentration, above 0.2 M KCl. Upon dialysis, the complex reformed, allowing further purification of its constituents. Three major protein bands were found, corresponding to proteins of the outer membrane. The complex did not sediment with membrane fractions, but adhered to the outer membrane in the presence of magnesium.
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