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. 1980 Feb;77(2):1106-10.
doi: 10.1073/pnas.77.2.1106.

Organization of kappa light chain genes in germ-line and somatic tissue

R Joho, I L Weissman, P Early, J Cole, L Hood

Organization of kappa light chain genes in germ-line and somatic tissue

R Joho et al. Proc Natl Acad Sci U S A. 1980 Feb.

Abstract

We studied the organization of the kappa light chain genes in germ-line (sperm) and somatic (embryo) tissues. We constructed a plasmid containing a DNA insert coding for the kappa chain MOPC 167 and used the Southern blotting technique to determine the organization of kappa variable and constant region genes. In the haploid genome of the mouse there is only one constant region gene detectable and it has the same organization in sperm and embryo DNAs. There are several variable region genes in sperm and embryo that are related to the Vk167 gene. The organization of the V genes in sperm and embryo DNAs is identical. These results show that there is no rearrangement of variable region genes (or "minigenes") during early embryogenesis.

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References

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