Identification and mapping of a second proline permease Salmonella typhimurium
- PMID: 6245058
- PMCID: PMC293781
- DOI: 10.1128/jb.141.3.1064-1070.1980
Identification and mapping of a second proline permease Salmonella typhimurium
Abstract
In this paper we demonstrate the existence of a second proline permease, gene proP, in Salmonella typhimurium. Uptake assays demonstrate that this second proline permease has 5 to 10% the uptake rate of the putP permease, the cell's major proline permease, when assayed at 20 microM proline. Genetic mapping by Hfr and P22-mediated genetic crosses placed the second proline permease gene at 92 min on the S. typhimurium genetic map, near the genes for melibiose utilization. F'-mediated complementation tests indicated that Escherichia coli also has the proP gene.
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