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. 1980 Jan 15;186(1):177-81.
doi: 10.1042/bj1860177.

A simple and fast method for the isolation of basolateral plasma membranes from rat small-intestinal epithelial cells

V Scalera, C Storelli, C Storelli-Joss, W Haase, H Murer

A simple and fast method for the isolation of basolateral plasma membranes from rat small-intestinal epithelial cells

V Scalera et al. Biochem J. .

Abstract

A method was developed for the analytical and preparative isolation of basolateral plasma membranes from rat small intestine. They were separated on a self-orientating Percoll (modified colloidal silica) gradient starting with a heavy microsomal-membrane fraction and involving centrifugation at 48,000 g for 1 h. (Na+ + K+)-stimulated ATPase activity, used as a marker enzyme for the basolateral plasma membrane, is enriched 20-fold compared with that found in the homogenate of isolated intestinal epithelial cells.

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