doi: 10.1111/j.1432-1033.1980.tb04470.x.
A DNA-binding protein specific for the early replicated region of the chromosome obtained from Escherichia coli membrane fractions
- PMID: 6245880
- DOI: 10.1111/j.1432-1033.1980.tb04470.x
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A DNA-binding protein specific for the early replicated region of the chromosome obtained from Escherichia coli membrane fractions
Eur J Biochem.
1980 Mar.
Free article
Abstract
After extensive dialysis of Escherichia coli membranes treated with Triton X-100, three membrane proteins (A, B and B') with an affinity for DNA have been isolated and purified. They bind to either double-stranded or single-stranded DNA. A and B' proteins preferentially attach to DNA even in the presence of poly(uridylic acid). Only protein B' can recognize some base sequence of DNA because pulse-labelled DNA made at the initiation of replication in a synchronized dnaC mutant has been selectively retained by the protein.
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