Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 Apr;34(1):280-4.
doi: 10.1128/JVI.34.1.280-284.1980.

Cell-free translation of avian erythroblastosis virus RNA

T Pawson, G S Martin

Cell-free translation of avian erythroblastosis virus RNA

T Pawson et al. J Virol. 1980 Apr.

Abstract

Avian erythroblastosis virus (AEV) RNA rescued from nonproducer cells by superinfection with a helper virus is translated into three polypeptides in the messenger-dependent rabbit reticulocyte lysate. A 75,000 molecular weight polypeptide (P75AEV) is synthesized from 28S RNA and is encoded by the 5' section of the AEV RNA, including gag-related and AEV-specific sequences. The P75AEV synthesized in infected cells and the P75AEV synthesized in the cell-free system are electrophoretically identical. A 44,000 molecular weight polypeptide (P44AEV) is synthesized from 20-24S RNA, apparently from the 3' section of the AEV-specific RNA sequence. A minor 37,000 molecular weight polypeptide (P37AEV) is synthesized from 20S AEV RNA. A comparison is drawn between the cell-free products of MC29 and AEV RNAs.

PubMed Disclaimer

References

    1. J Natl Cancer Inst. 1967 Mar;38(3):251-85 - PubMed
    1. J Mol Biol. 1975 Aug 15;96(3):471-93 - PubMed
    1. Virology. 1976 Jun;71(2):423-33 - PubMed
    1. Eur J Biochem. 1976 AUG 1;67(1):247-56 - PubMed
    1. Virology. 1977 Oct 15;82(2):431-48 - PubMed

Publication types

LinkOut - more resources