Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 May 22;285(5762):207-10.
doi: 10.1038/285207a0.

Isolation of the chicken thymidine kinase gene by plasmid rescue

M Perucho, D Hanahan, L Lipsich, M Wigler

Isolation of the chicken thymidine kinase gene by plasmid rescue

M Perucho et al. Nature. .

Abstract

We have used the bacterial plasmid pBR322 as a vehicle to isolate genes coding for selectable markers from higher eukaryotes. In this way, we have obtained the chicken thymidine kinase (tk) gene as a 2.2-kilobase EcoRI/HindIII insert in BR322. The cloned gene transforms tk- animal cells with an efficiency equal to that of the cloned herpes simplex virus-1 tk gene.

PubMed Disclaimer

Publication types

LinkOut - more resources