Sialic acids in bone demineralised in organic solvents
- PMID: 6246870
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Sialic acids in bone demineralised in organic solvents
Basic Appl Histochem.
1980.
Abstract
The sialic acid content of bovine shin bone, demineralised by a new process in ethanol-trimetrylammonium EDTA, was measured and compared with that of the original whole bone. New variants of the direct Ehrlich procedure, as well as the periodate-thiobarbituric acid assay, were employed. About 75% of the sialic acid remains in the bone matrix from ethanolic demineralisation, and the PAS reaction is correspondingly much more intense, compared with matrix from aqueous EDTA demineralisation.
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