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. 1980;24(1):17-21.

Sialic acids in bone demineralised in organic solvents

J E Scott, T W Kyffin
  • PMID: 6246870

Sialic acids in bone demineralised in organic solvents

J E Scott et al. Basic Appl Histochem. 1980.

Abstract

The sialic acid content of bovine shin bone, demineralised by a new process in ethanol-trimetrylammonium EDTA, was measured and compared with that of the original whole bone. New variants of the direct Ehrlich procedure, as well as the periodate-thiobarbituric acid assay, were employed. About 75% of the sialic acid remains in the bone matrix from ethanolic demineralisation, and the PAS reaction is correspondingly much more intense, compared with matrix from aqueous EDTA demineralisation.

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