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. 1980 Jan 1;185(1):59-63.
doi: 10.1042/bj1850059.

The isolation and characterization of a sequence-specific endonuclease from Anabaena subcylindrica

S G Hughes, T Bruce, K Murray

The isolation and characterization of a sequence-specific endonuclease from Anabaena subcylindrica

S G Hughes et al. Biochem J. .

Abstract

An endonuclease, AsuI, was isolated from extracts of Anabaena subcylindrica on the basis of gel-electrophoretic analysis of digests of bacteriophage-lambda DNA with the paritally purified extracts. The enzyme requires Mg2+, but no other cofactors. Endonuclease AsuI recognizes the interrupted tetranucleotide sequence: (Formula: see tex), and breaks the phosphodiester bonds indicated by the arrows to leave single-stranded trinucleotide projections at the 5'-termini of the DNA fragments.

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