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. 1980 May;142(2):380-3.
doi: 10.1128/jb.142.2.380-383.1980.

RsaI: a new sequence-specific endonuclease activity from Rhodopseudomonas sphaeroides

S P Lynn, L K Cohen, S Kaplan, J F Gardner

RsaI: a new sequence-specific endonuclease activity from Rhodopseudomonas sphaeroides

S P Lynn et al. J Bacteriol. 1980 May.

Abstract

A new type II sequence-specific endonuclease, RsaI, has been identified from Rhodopseudomonas sphaeroides strain 28/5. An RsaI purification scheme that yields enzyme which is free of contaminating exonuclease and phosphatase activities after a single column fractionation has been developed. The enzyme recognized the tetranucleotide sequence 5'-GTAC-3' and cleaved between the T and A, thereby generating flush ends. RsaI should be extremely useful in deoxyribonucleic acid sequencing experiments.

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