doi: 10.1007/BF00272674.
The boundaries and copy numbers of Ti plasmid T-DNA vary in crown gall tumors
- PMID: 6247611
- DOI: 10.1007/BF00272674
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The boundaries and copy numbers of Ti plasmid T-DNA vary in crown gall tumors
Mol Gen Genet.
1980.
Abstract
The Ti plasmid DNA maintained in octopine-type crown gall tumor lines is variable, but always includes at least part of the Ti plasmid that maps over the region of Hind III fragment 1 of pTiB6-806. The right-hand boundary of transferred DNA (T-DNA) varies considerably among the three independent tumor lines examined; the left boundary was not located definitively. The T-DNA of two sibling clones of the same tumor line, E1 and E9, appears identical. The copy number of T-DNA in E9 tumor DNA appears higher for the right end (about 30 copies) than for the left end (approximately 1 copy).
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