Electron microscopic mapping and sequence analysis of the terminator for the early message of E. coli phage T7

Abstract

The terminator position of T7 early messenger RNA was determined by electron microscopic measurements. The end of the RNA was mapped at a position 18.9% from the left end of T7 DNA, and 145 +/- 25 nucleotides from the right end of the Hpa I fragment Q. The sequence of the Hpa I Q fragment was determined around this position, and a terminator-like structure was detected in position 193 to 169 from the right end of fragment Q.