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. 1978;19(2-3):169-72.
doi: 10.1016/0022-1759(78)90176-x.

A simple method for the isolation of murine peripheral blood lymphocytes

D S Chi, N S Harris

A simple method for the isolation of murine peripheral blood lymphocytes

D S Chi et al. J Immunol Methods. 1978.

Abstract

A simple, rapid and reproducible semi-microtechnique was developed for the isolation of murine peripheral blood lymphocytes. Murine peripheral blood was collected from brachial vessels and centrifuged through a Ficoll--Hypaque gradient in a microfuge tube yielding 1--1.5 X 10(6) lymphocytes per mouse.

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