Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980;178(2):271-9.
doi: 10.1007/BF00270472.

Genetics and physiology of the rel system of Bacillus subtilis

I Smith, P Paress, K Cabane, E Dubnau

Genetics and physiology of the rel system of Bacillus subtilis

I Smith et al. Mol Gen Genet. 1980.

Abstract

Stringent factor (ATP:GTP-3' pyrophosphotransferase) has been purified from wild type Bacillus subtilis and it has been shown that guanosine tetra- and pentaphosphate (ppGpp and pppGpp) are synthesized in vitro in the presence of ribosomes, unacylated tRNA and its specific codon, as has been demonstrated in Escherichia coli. relA, the genetic determinant for the stringent factor, has been mapped on the B. subtilis chromosome by transduction and is found between aroD and leu. The relC locus, defined by mutations which were originally selected by resistance to thiostrepton, has been mapped adjacent to spoOH in the order cysA, spoOH, relC, rif. Sringent factor and ribosomes are functional for the in vitro synthesis of (p)ppGpp in early stages of sporulation (up to at least 4 h). This contradicts the findings of other laboratories.

PubMed Disclaimer

References

    1. Biken J. 1972 Jun;15(2):81-97 - PubMed
    1. Nature. 1969 Mar 1;221(5183):838-41 - PubMed
    1. J Mol Biol. 1969 Oct 28;45(2):155-79 - PubMed
    1. Eur J Biochem. 1976 Apr 15;64(1):205-13 - PubMed
    1. J Biol Chem. 1972 Apr 25;247(8):2289-94 - PubMed

Publication types

LinkOut - more resources