Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 May;77(5):2979-83.
doi: 10.1073/pnas.77.5.2979.

Monoclonal antibody against a 250,000-dalton glycoprotein of Epstein-Barr virus identifies a membrane antigen and a neutralizing antigen

G J Hoffman, S G Lazarowitz, S D Hayward

Monoclonal antibody against a 250,000-dalton glycoprotein of Epstein-Barr virus identifies a membrane antigen and a neutralizing antigen

G J Hoffman et al. Proc Natl Acad Sci U S A. 1980 May.

Abstract

An antibody-secreting hybrid cell line was produced by fusion of mouse myeloma cells with splenocytes from mice immunized with virions of the B95-8 strain of Epstein-Barr Virus (EBV). The monoclonal IgG antibody was shown to have anti-EBV activity by the following criteria: (i) It reacted with the membranes and the cytoplasm of seven different EBV-producing lines, but with no nonproducing line. (ii) The individual cells identified by the murine antibody were shown to be the same cells identified by a human serum having anti-EBV activity. (iii) The antibody significantly reduced the infectivity of two independent strains of EBV (namely, P3HR1K and B95-8). The antigen being recognized was characterized by immunoprecipitations of radiolabeled EBV-producer cell lysates. A single glycoprotein with an estimated molecular weight of 250,000 was identified. It is concluded that neutralization of EBV can be achieved by an IgG-class monoclonal antibody directed against a single antigenic site on a 250,000-dalton glycoprotein, which is a constituent of the EBV virion.

PubMed Disclaimer

References

    1. Virology. 1969 Oct;39(2):312-21 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Gen Virol. 1972 Nov;17(2):233-6 - PubMed
    1. Eur J Biochem. 1974 Jul 1;46(1):83-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4006-10 - PubMed

Publication types