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. 1980 Jun;11(3-4):439-42.
doi: 10.1016/0301-4622(80)87020-7.

Dynamic light scattering studies of ribonuclease

C C Wang, K H Cook, R Pecora

Dynamic light scattering studies of ribonuclease

C C Wang et al. Biophys Chem. 1980 Jun.

Abstract

Dynamic light scattering has been used to measure the translational diffusion coefficients of bovine pancreatic ribonuclease A as functions of temperature and concentration in the presence of 1 M Guanidine-HCl. Data was collected throughout a temperature range including the folding-unfolding transitions. Evidence of a pretransition "swelling" of the protein was observed. Entropy and enthalpy changes upon unfolding were obtained using a two-state model.

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