Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1980 Jul;49(1):83-9.
doi: 10.1099/0022-1317-49-1-83.

Enzyme-linked immunosorbent assay for coronaviruses HCV 229E and MHV 3

C A Kraaijeveld, M H Madge, M R Macnaughton
Comparative Study

Enzyme-linked immunosorbent assay for coronaviruses HCV 229E and MHV 3

C A Kraaijeveld et al. J Gen Virol. 1980 Jul.

Abstract

The antigenic relationship between human cornonavirus strain 229E (HCV 229E) and mouse hepatitis virus strain 3 (MHV 3) was studied by means of the indirect form of enzyme-linked immunosorbent assay (ELISA). A cross-reaction was found with hyprimmune rabbit sera between HCV 229E and MHV 3 which may be due to the adherence of bovine serum componeants from tissue culture media, which were present on virus particles even after extensive purification. No cross-reaction was observed with immune sera absorbed with bovine serum, or with HCV 229E grown in tissue culture without serum. This indirect ELISA with HCV 229E may prove to be useful for studies with human sera.

PubMed Disclaimer

Publication types

LinkOut - more resources