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. 1980 Oct 10;8(19):4327-35.
doi: 10.1093/nar/8.19.4327.

Fusion of the promoter region of rRNA operon rrnB to lac Z gene

Free PMC article

Fusion of the promoter region of rRNA operon rrnB to lac Z gene

G Glaser et al. Nucleic Acids Res. .
Free PMC article

Abstract

A Lambda phage was constructed in which the structural gene for beta galactosidase is fused to a DNA segment carrying the ribosomal promoter rrnB of E. coli. In this hybrid operon beta galactosidase synthesis in vitro is repressed by ppGpp. Repression of beta galactosidase synthesis by cAMP is reported.

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