Fusion of the promoter region of rRNA operon rrnB to lac Z gene
- PMID: 6253913
- PMCID: PMC324242
- DOI: 10.1093/nar/8.19.4327
Fusion of the promoter region of rRNA operon rrnB to lac Z gene
Abstract
A Lambda phage was constructed in which the structural gene for beta galactosidase is fused to a DNA segment carrying the ribosomal promoter rrnB of E. coli. In this hybrid operon beta galactosidase synthesis in vitro is repressed by ppGpp. Repression of beta galactosidase synthesis by cAMP is reported.
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