doi: 10.1093/nar/8.19.4501.
An Escherichia coli endonuclease responsible for primary cleavage of in vitro transcripts of bacteriophage T4 tRNA gene cluster
- PMID: 6253921
- PMCID: PMC324254
- DOI: 10.1093/nar/8.19.4501
Item in Clipboard
An Escherichia coli endonuclease responsible for primary cleavage of in vitro transcripts of bacteriophage T4 tRNA gene cluster
Nucleic Acids Res.
.
Free PMC article
Abstract
An endonuclease activity was isolated from 100,000 g supernatant fraction of Escherichia coli using in vitro primary transcripts of T4 tRNA gene cluster as assay substrates. The endonuclease cleaves the polycistronic RNA precursors into fragments containing monomeric and dimeric stable RNA sequences. The result strongly suggest that this enzyme participates in the early steps of T4 tRNA maturation pathway preceding the action of RNase P.
Similar articles
-
IN vitro transcription of bacteriophage T4 tRNA gene cluster from two different promoters.Nucleic Acids Res. 1981 Feb 11;9(3):519-27. doi: 10.1093/nar/9.3.519. Nucleic Acids Res. 1981. PMID: 7220346 Free PMC article.
-
Biosynthesis of transfer RNA.CRC Crit Rev Biochem. 1981;9(4):253-92. doi: 10.3109/10409238109105436. CRC Crit Rev Biochem. 1981. PMID: 6258859 Review. No abstract available.
-
Mapping of transcription units in the bacteriophage T4 tRNA gene cluster.J Mol Biol. 1981 Mar 15;146(4):393-412. doi: 10.1016/0022-2836(81)90039-5. J Mol Biol. 1981. PMID: 7024554 No abstract available.
-
DNA sequence of the transfer RNA region of bacteriophage T4: implications for transfer RNA synthesis.Proc Natl Acad Sci U S A. 1981 Feb;78(2):889-92. doi: 10.1073/pnas.78.2.889. Proc Natl Acad Sci U S A. 1981. PMID: 6262781 Free PMC article.
-
[Processing of tRNA].Mol Biol (Mosk). 1981 Jan-Feb;15(1):5-26. Mol Biol (Mosk). 1981. PMID: 7038444 Review. Russian.
Cited by
-
Nucleolytic processing of ribonucleic acid transcripts in procaryotes.Microbiol Rev. 1986 Dec;50(4):428-51. doi: 10.1128/mr.50.4.428-451.1986. Microbiol Rev. 1986. PMID: 2432388 Free PMC article. Review. No abstract available.
-
Identification and location of nine T5 bacteriophage tRNA genes by DNA sequence analysis.Nucleic Acids Res. 1986 May 27;14(10):4197-205. doi: 10.1093/nar/14.10.4197. Nucleic Acids Res. 1986. PMID: 3012464 Free PMC article.
-
Dual function transcripts specifying tRNA and mRNA.Nature. 1981 Dec 3;294(5840):422-7. doi: 10.1038/294422a0. Nature. 1981. PMID: 7312036 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
