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Comparative Study
. 1980 Mar 25;8(6):1187-99.
doi: 10.1093/nar/8.6.1187.

Complete nucleotide sequence of a cloned chicken alpha-globin cDNA

N J Deacon, J Shine, H Naora
Free PMC article
Comparative Study

Complete nucleotide sequence of a cloned chicken alpha-globin cDNA

N J Deacon et al. Nucleic Acids Res. .
Free PMC article

Abstract

Chicken globin double-stranded cDNA was synthesised from anaemic adult reticulocyte alpha- and beta-globin mRNA and ligated into the Hind III site of pBR322 using synthetic Hind III decamers. Transformation of E. coli x1776 resulted in the production of a number of alpha- and beta-cDNA clones. One of the alpha-type clones (pCG alpha-8) was fully sequenced and found to code for neither alpha A- nor alpha D-globin. Partial sequencing of the other alpha-cDNA clones indicates that they are all of the same type.

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References

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