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. 1980 Mar 25;8(6):1373-81.
doi: 10.1093/nar/8.6.1373.

Endonucleases for UV-irradiated and depurinated DNA in barley chloroplasts

J Velemínský, J Svachulová, J Satava
Free PMC article

Endonucleases for UV-irradiated and depurinated DNA in barley chloroplasts

J Velemínský et al. Nucleic Acids Res. .
Free PMC article

Abstract

Lysates of barley chloroplasts release more radioactivity into acid soluble form from UV-irradiated and alkylated-depurinated E. coli [3H] DNA than from intact DNA. By means of affinity chromatography on depurinated DNA-cellulose and/or UV irradiated DNA-cellulose and by electrophoresis in polyacrylamide gels, four activities on depurinated DNA were separated. One of these contained activity against heavily UV-irradiated /270 J.m-2/ native DNA. In addition, two other nucleases specific towards UV-DNA were separated. One of them was active on native and heat denatured DNA irradiated with 10 J . m-2 UV, whereas the other was predominantly active on native UV-irradiated DNA.

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