Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 Mar 25;8(6):1373-81.
doi: 10.1093/nar/8.6.1373.

Endonucleases for UV-irradiated and depurinated DNA in barley chloroplasts

J Velemínský, J Svachulová, J Satava
Free PMC article

Endonucleases for UV-irradiated and depurinated DNA in barley chloroplasts

J Velemínský et al. Nucleic Acids Res. .
Free PMC article

Abstract

Lysates of barley chloroplasts release more radioactivity into acid soluble form from UV-irradiated and alkylated-depurinated E. coli [3H] DNA than from intact DNA. By means of affinity chromatography on depurinated DNA-cellulose and/or UV irradiated DNA-cellulose and by electrophoresis in polyacrylamide gels, four activities on depurinated DNA were separated. One of these contained activity against heavily UV-irradiated /270 J.m-2/ native DNA. In addition, two other nucleases specific towards UV-DNA were separated. One of them was active on native and heat denatured DNA irradiated with 10 J . m-2 UV, whereas the other was predominantly active on native UV-irradiated DNA.

PubMed Disclaimer

References

    1. Cold Spring Harb Symp Quant Biol. 1968;33:209-18 - PubMed
    1. Biochemistry. 1973 Mar 13;12(6):1202-7 - PubMed
    1. Nature. 1975 Mar 13;254(5496):160-1 - PubMed
    1. J Biol Chem. 1976 Mar 10;251(5):1438-45 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1169-73 - PubMed