Cloning of V region fragments from mouse liver DNA and localization of repetitive DNA sequences in the vicinity of immunoglobulin gene segments
- PMID: 6253945
- PMCID: PMC324031
- DOI: 10.1093/nar/8.8.1721
Cloning of V region fragments from mouse liver DNA and localization of repetitive DNA sequences in the vicinity of immunoglobulin gene segments
Abstract
Two different kappa light chain genes have previously been isolated from one mouse myeloma. The V (variable, abbreviations in ref. 2) gene segments of the two genes were now used to identify their germline counterparts in EcoRI digests of mouse liver DNA. In addition two sets of related V gene segments were found which hybridize with either of the two DNA probes. Five of the V region fragments of one set were cloned in a lambda phage vector and partially characterized by restriction mapping and Southern blot hybridization. Repetitive DNA sequences were found on each of the five fragments as well as on other cloned immunoglobulin gene containing fragments. Cross-hybridization between some but not all of the regions containing repetitive DNA sequences was observed.
Similar articles
-
The nucleotide sequences of rearranged and germline immunoglobulin VH genes of a mouse myeloma MC101 and evolution of VH genes in mouse.J Biol Chem. 1982 Jan 10;257(1):277-85. J Biol Chem. 1982. PMID: 6273429 No abstract available.
-
Sets of immunoglobulin V kappa genes homologous to ten cloned V kappa sequences: implications for the number of germline V kappa genes.J Mol Appl Genet. 1981;1(2):103-16. J Mol Appl Genet. 1981. PMID: 6286818
-
A rearranged DNA sequence possibly related to the translocation of immunoglobulin gene segments.Nucleic Acids Res. 1980 Apr 25;8(8):1709-20. doi: 10.1093/nar/8.8.1709. Nucleic Acids Res. 1980. PMID: 6253944 Free PMC article.
-
Cloning of immunoglobulin kappa light chain genes from mouse liver and myeloma MOPC 173.Nucleic Acids Res. 1979 Jul 25;6(10):3213-29. doi: 10.1093/nar/6.10.3213. Nucleic Acids Res. 1979. PMID: 113775 Free PMC article.
-
A new era in mammalian gene mapping: somatic cell genetics and recombinant DNA methodologies.Nature. 1981 Nov 12;294(5837):115-20. doi: 10.1038/294115a0. Nature. 1981. PMID: 6272118 Review.
Cited by
-
Preferential rearrangement of V kappa 4 gene segments in pre-B cell lines.J Exp Med. 1990 Aug 1;172(2):559-66. doi: 10.1084/jem.172.2.559. J Exp Med. 1990. PMID: 2115571 Free PMC article.
-
A new site of integration for mouse mammary tumor virus proviral DNA common to BALB/cf(C3H) mammary and kidney adenocarcinomas.EMBO J. 1986 Jan;5(1):127-34. doi: 10.1002/j.1460-2075.1986.tb04186.x. EMBO J. 1986. PMID: 3007110 Free PMC article.
-
DNA organisation in the chicken lysozyme gene region.Nucleic Acids Res. 1981 Aug 11;9(15):3575-88. doi: 10.1093/nar/9.15.3575. Nucleic Acids Res. 1981. PMID: 6269085 Free PMC article.
-
Recombined flanks of the variable and joining segments of immunoglobulin genes.Proc Natl Acad Sci U S A. 1982 Mar;79(5):1383-7. doi: 10.1073/pnas.79.5.1383. Proc Natl Acad Sci U S A. 1982. PMID: 6803241 Free PMC article.
-
Two rearranged immunoglobulin kappa light chain genes in one mouse myeloma.Nucleic Acids Res. 1980 Apr 25;8(8):1693-707. doi: 10.1093/nar/8.8.1693. Nucleic Acids Res. 1980. PMID: 6253943 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
