Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 Apr 25;8(8):1731-43.
doi: 10.1093/nar/8.8.1731.

A short primer for sequencing DNA cloned in the single-stranded phage vector M13mp2

S Anderson, M J Gait, L Mayol, I G Young
Free PMC article

A short primer for sequencing DNA cloned in the single-stranded phage vector M13mp2

S Anderson et al. Nucleic Acids Res. .
Free PMC article

Abstract

In this paper we describe the synthesis and cloning of a short segment of DNA complementary to the region immediately adjacent to the EcoRI insertion site in the single-stranded bacteriophage vector M13mp2. This segment is useful as a "universal" primer for DNA sequencing by the dideoxynucleotide chain termination method; the template can be any DNA species cloned in M13mp2 or its derivatives. The primer has been cloned into the tetracycline resistance gene of plasmid pBR322 as one strand of a 26 bp EcoRI/BamHI fragment. This fragment may be readily prepared from an EcoRI + BamHI restriction digest of the parent plasmid (designated pSP14) by a simple size fractionation.

PubMed Disclaimer

References

    1. J Biol Chem. 1968 Sep 10;243(17):4530-42 - PubMed
    1. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3117-21 - PubMed
    1. Nucleic Acids Res. 1976 Oct;3(10):2575-92 - PubMed

Publication types