Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 Jul;77(7):3841-4.
doi: 10.1073/pnas.77.7.3841.

Calmodulin activates prokaryotic adenylate cyclase

J Wolff, G H Cook, A R Goldhammer, S A Berkowitz

Calmodulin activates prokaryotic adenylate cyclase

J Wolff et al. Proc Natl Acad Sci U S A. 1980 Jul.

Abstract

The adenylate cyclase of Bordetella pertussis is stimulated 100- to 1000-fold in a dose-dependent manner by calf brain calmodulin. The system has the following properties. (i) The activation is prevented by ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid and restored by Ca2+. (ii) Oxidation of the methionine residues of calmodulin abolishes the ability to activate the cyclase. (iii) Trifluoperazine inhibits calmodulin-activated cyclase. (iv) A troponin C preparation stimulates the B. pertussis cyclase with < 0.01 the potency of calmodulin. Although calmodulin has not been demonstrated in prokaryotes, this is an example of a (eukaryotic) calmodulin effect in a prokaryote.

PubMed Disclaimer

References

    1. Int Rev Neurobiol. 1966;9:145-221 - PubMed
    1. Proc Natl Acad Sci U S A. 1970 Feb;65(2):454-9 - PubMed
    1. Anal Biochem. 1974 Apr;58(2):541-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Jan;72(1):64-8 - PubMed
    1. Biochem Biophys Res Commun. 1975 Oct 6;66(3):1055-62 - PubMed

MeSH terms

LinkOut - more resources