doi: 10.1073/pnas.77.7.3870.
Transport of vesicular stomatitis virus glycoprotein in a cell-free extract
- PMID: 6253996
- PMCID: PMC349728
- DOI: 10.1073/pnas.77.7.3870
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Transport of vesicular stomatitis virus glycoprotein in a cell-free extract
Proc Natl Acad Sci U S A.
1980 Jul.
Abstract
We describe a cell-free system in which the membrane glycoprotein of vesicular stomatitis virus is rapidly and efficiently transported to membranes of the Golgi complex by a process resembling intracellular protein transport. Transport in vitro is energy-dependent and is accompanied by terminal glycosylation of the membrane glycoprotein (dependent upon UDP-GlcNAc and resulting in resistance to endo-beta-N-acetylglucosaminidase H).
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