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. 1980 Aug;77(8):4662-5.
doi: 10.1073/pnas.77.8.4662.

Polycistronic vesicular stomatitis virus RNA transcripts

R C Herman, M Schubert, J D Keene, R A Lazzarini

Polycistronic vesicular stomatitis virus RNA transcripts

R C Herman et al. Proc Natl Acad Sci U S A. 1980 Aug.

Abstract

A procedure to enrich for the sequences present at the junction between the linked messages in the polycistronic RNAs symthesized in vitro by vesicular stomatitis virus (VSV) is described. Analyses of these sequences show that they contain a precise transcript of both the intercistronic dinucleotide and the pentanucleotide 5'--C-U-G-U-U--3', common to the 5'-end of all VSV cistrons, covalently linked to the 3'-side of the intervening poly(A). The data strongly suggest that the VSV transcriptase polyadenylylates the mRNAs and can then resume direct and precise transcription of the genome-without reinitiation and without skipping nucleotides.

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References

    1. J Mol Biol. 1965 Sep;13(2):373-98 - PubMed
    1. Virology. 1970 Aug;41(4):615-30 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3360-4 - PubMed
    1. Nat New Biol. 1972 Nov 8;240(97):43-5 - PubMed
    1. Cell. 1974 Sep;3(1):77-84 - PubMed

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