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. 1980 Oct;11(1-2):169-71.
doi: 10.1016/0378-1119(80)90097-9.

A cautionary note on the use of certain restriction endonucleases with methylated substrates

K Backman

A cautionary note on the use of certain restriction endonucleases with methylated substrates

K Backman. Gene. 1980 Oct.

Abstract

Methylation of adenine and cytosine residues in DNA isolated from common strains of Escherichia coli K-12 can render that DNA resistant to cleavage by certain restriction endonucleases at those sites at which the recognition sequence for such an endonuclease overlaps (but does not include) a sequence recognized by methylases specified by the dam or dcm gene.

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