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. 1980 Oct;11(1-2):53-62.
doi: 10.1016/0378-1119(80)90086-4.

Nucleotide sequence of a cloned fragment of rat mitochondrial DNA containing the replication origin

T Sekiya, M Kobayashi, T Seki, K Koike

Nucleotide sequence of a cloned fragment of rat mitochondrial DNA containing the replication origin

T Sekiya et al. Gene. 1980 Oct.

Abstract

The nucleotide sequence was determined for the 717 bp HapII subfragment (HapEcoA5) of the EcoRI-A fragment of rat mitochondrial DNA, which contains the heavy-strand replication origin. Analysis of the heavy-strand initiation segments released from the D-loop molecules has revealed that some 5'-ends of these initiation segments are linked to ribonucleotide(s) and are heterogeneous. Sequence analysis of the 5'-end portion of the initiation segment indicated that one of the start points of the deoxyribonucleotide polymerization corresponds to the 425th bp on the HapEcoA5. Two-fold rotational symmetry and palindrome structures, and a G-cluster sequence around the start point have been discussed in connection with unidirectional replication.

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