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. 1980 Dec;144(3):877-83.
doi: 10.1128/jb.144.3.877-883.1980.

Formation of a lambda (Tn10) tyrR+ specialized transducing bacteriophage from Escherichia coli K-12

C S Cobbett, J Pittard

Formation of a lambda (Tn10) tyrR+ specialized transducing bacteriophage from Escherichia coli K-12

C S Cobbett et al. J Bacteriol. 1980 Dec.

Abstract

The transposon Tn10, coding for resistance to tetracycline, was inserted close to the tyrR+ gene at min 28 on the Escherichia coli chromosome. The homology between this transposon and a lambda (Tn10) phage was employed to direct integration of lambda close to tyrR+ with subsequent isolation of a lambda (Tn10) tyrR+ transducing phage. Results of restriction endonuclease analysis of the transducing phage are presented.

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References

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