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. 1980 Sep;50(1):59-68.
doi: 10.1099/0022-1317-50-1-59.

Detection of IgM antibodies to cytomegalovirus (CMV) using an enzyme-labelled antigen (ELA)

H Schmitz, U von Deimling, B Flehmig

Detection of IgM antibodies to cytomegalovirus (CMV) using an enzyme-labelled antigen (ELA)

H Schmitz et al. J Gen Virol. 1980 Sep.

Abstract

We have applied a peroxidase enzyme-labelled antigen (ELA) for the detection of IgM antibodies to cytomegalovirus: microtitre plates were coated with anti-IgM immunoglobulin. The IgM fraction of human serum was selectively bound to the precoated plates and the virus-specific IgM antibody was then detected by the enzyme-labelled antigen. A very efficient technique for the labelling of virus antigen is described. The IgM antibody was detected simply and specifically, Rheumatoid factor IgM did not interfere with this test.

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