An immunocytochemist's view of gonadotropin storage in the adult male rat: cytochemical and morphological heterogeneity in serially sectioned gonadotropes

Abstract

This study was designed to elucidate hormone storage patterns in gonadotropes with the use of ultrastructural immunocytochemistry on serial ultrathin sections. Sets of six serial sections were strained for beta chains of LH, FSH, or the C-Terminal sequence of ACTH, and 430 cells cut in triple or double serial section were collected from a group of seven normal adult male rats. Approximately 50--80% of the cells contained both LH and FSH, and most of these were Type I cells which are distinguished by their round shape and heterogeneous populations of secretion granules. Cells containing only FSH or LH constituted, on average, 19% of the population. These were a mixed group, morphologically, and included Type II cells distinguished by their angular shape and population of secretion granules, 250 nm in average diameter. Also among the FSH cells (and a few LH cells in two of the rats) were Type III cells, which resemble the corticotrope. On average, 10% of the serially sectioned cells contained only ACTH. Our findings show the presence of subpopulations of gonadotropes containing only one of the hormones, in numbers large enough to support the hypothesis that they may be partly responsible for the nonparallel release of gonadotropins. Also, the FSH-LH cells seemed to vary in their staining intensity for the two hormones, suggesting that the gondaotropes are a fluid, heterogeneous population of cells capable of storing both or only one of the hormones.