doi: 10.1038/289378a0.
A gene chimaera of SV40 and mouse beta-globin is transcribed and properly spliced
- PMID: 6258077
- DOI: 10.1038/289378a0
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A gene chimaera of SV40 and mouse beta-globin is transcribed and properly spliced
Nature.
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Abstract
A gene chimaera of the first exon from simian virus 40 (SV40) sequences coding for T antigen placed upstream from the third exon from mouse beta-globin, and separated by the resultant new chimaeric intron, was cloned into a bacterial plasmid. When transfected into monkey cells, the gene chimaera was transcribed, polyadenylated and spliced using the donor splice site from SV40 and the acceptor splice site from mouse beta-globin. This result suggests that a donor site from one gene can be spliced to an acceptor site from another gene.
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